titleabstractauthorfull-authorpmidyearjournalmeshtitleabstractcontentswheat LR* (resistance to pathogen)wheatLRresistancetopathogenwheatlr{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/ +((title:wheat abstract:wheat)~1) +((title:lr* abstract:lr*)~1) +((title:{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/* abstract:{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/*)~1) pathogen resistancedisease and/or pathogen resistancedisease resistancedisease-resistanceresistance to a pathogenresistance to a plant pathogenresistance to diseaseresistance to infectionresistance to pathogensresistance to the plant pathogen{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031089/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0030998/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0030998/wp:0000015/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031089/wp:0000000/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0030909/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000063/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0030998/wp:0000007/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0030998/wp:0000008/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/wp:0000009/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/wp:0000012/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000149/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000126/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000318/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000147/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000153/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0030905/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000137/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000383/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000162/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000156/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000150/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031089/id:0000297/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031089/wp:0000002/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0030998/id:0031123/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0030998/id:0031007/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0031161/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0030998/id:0031164/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/wp:0000021/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000085/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000130/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000321/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0030998/id:0000180/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0030998/id:0000179/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031089/wp:0000001/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031089/id:00000097/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031089/id:00000004/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:00000143/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000126/id:0000127/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/wp:0000021/id:0000278/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0031161/id:0031133/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000126/id:0000122/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/wp:0000021/id:0000160/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000126/id:0000101/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000085/id:0031017/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0031161/id:0030907/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/wp:0000021/wp:0000022/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/wp:0000021/id:0031219/{phenotype}/id:0000247/id:0000000/id:0000036/id:0000002/id:0000006/id:0030988/id:0031083/id:0000130/id:0000107/{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is known about the genetic impacts of modern plant breeding on specific breeding target loci. Resequencing cloned genes can identify all mutations in single genes for population-based analyses of genetic changes in improved gene pools. Ninety-five wheat cultivars released in Canada from 1845 to 2004 were sequenced at the wheat leaf rust resistance locus Lr21. Characterization of the DNA fragment of length 4,071 bp, covering the Lr21 gene from -92 to +4,261, revealed 13 SNPs, four indels, 10 haplotypes, and 4 major haplotype groups. A new SCAR marker was developed to identify the resistant haplotype and haplotype groups. Non-synonymous polymorphic sites and haplotype numbers were increased over the 100 years of wheat breeding. Nucleotide diversity of the wheat cultivars was gradually reduced from 1845 to 1993 and increased after the release of the first Lr21 wheat cultivar AC Cora in 1994. Positive selection measured with Tajima's D was observed in the cultivars released before 1935. At least two recombination events were inferred in those cultivars released before 1993. Linkage disequilibrium at the locus was decreased over time. These findings demonstrate not only the effectiveness of the wheat breeding in the improvement of leaf rust resistance, but also are useful to understand the genetic influences of a long-term artificial selection on individual loci.Fu, YBPeterson, GWMcCallum, BDHuang, LTheor. Appl. Genet.10.1007/s00122-010-1308-7Population-based resequencing analysis of improved wheat germplasm at wheat leaf rust resistance locus Lr212010Leaf rust is a widespread and commonly occurring rust disease of wheat. Genetic resistance is the most economical method of reducing losses due to leaf rust. Lr15 has been shown to be present on wheat chromosome 2D and is reported to be a seedling resistance gene. However, tightly linked markers associated with Lr15 have not been reported to date. To identify molecular markers linked to Lr15, an F-2 mapping population of Thatcher x Thatcher-Lr15 was generated. Available wheat simple sequence repeat markers were utilized in parental screening and polymorphic markers were used to analyze the entire population of 221 plants. Phenotypic evaluations of the F-2-derived F-3 progenies with Puccinia triticina Eriks. pathotype 162A (93R15) confirmed the monogenic inheritance of Lr15. The linkage group representing chromosome 2DS was constructed at LOD 4.0 which revealed the closest flanking markers Xgwm4562 and Xgwm102 at a distance of 3.1 and 9.3 cM, respectively. Furthermore, utilization of these flanking markers in combination has successfully identified wheat lines with or without Lr15. These markers could potentially be useful in gene pyramiding with other genes to enhance rust resistance in wheat.Dholakia, BBRajwade, AVHosmani, PKhan, RRChavan, SReddy, DMRLagu, MDBansal, UKSaini, RGGupta, VSMol. Breed.10.1007/s11032-012-9813-9Molecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat2013The Lr34/Yr18 adult plant resistance gene contributes significantly to durable leaf rust (caused by Puccinia triticina Eriks.) resistance. Simple and robust molecular markers that enable early detection of Lr34/Yr18 are a major advancement in wheat (Triticum aestivum L.) breeding. An insertion/deletion size variant located at the csLV34 locus on chromosome 7D within an intron sequence of a sulfate transporter-like gene tightly linked to the Lr34/Yr18 dual rust resistance gene was used to examine a global collection of wheat cultivars, landraces, and D genome-containing diploid and polyploid species of wheat relatives. Two predominant allelic size variants, csLV34a and b, found among the wheat cultivars showed disparate variation in different wheat growing zones. A strong association was observed between the presence of Lr34/Yr18 and the csLV34b allele and wheat lines known to have Lr34/Yr18 that had the csLV34a allele were rare. All landraces with the exception of those from China were predominantly of the csLV34a type. Only one size variant, csLV34a, was detected among the diploid and polyploid D genome-containing species, indicating that csLV34b arose subsequent to hexaploid bread wheat synthesis. The lineage of the csLV34b allele associated with Lr34/Yr18 in modern wheat cultivars from North and South America, CIMMYT, Australia, and Russia was tracked back to the cultivars Mentana and Ardito developed in Italy by Nazareno Strampelli in the early 1900s. The robustness of the csLV34 marker in postulating the likely occurrence of Lr34/Yr18 across a wide range of wheat germplasm and its utility in wheat breeding was confirmed.Kolmer, JASingh, RPGarvin, DFViccars, LWilliam, HMHuerta-Espino, JOgbonnaya, FCRaman, HOrford, SBariana, HSLagudah, ESCrop Sci.10.2135/cropsci2007.08.0474Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm2008Wheat leaf rust, caused by Puccinia triticina Eriks., infects millions of acres of wheat (Triticum aestivum L.) and causes leaves to senesce prematurely, which in turn decreases yield by reducing the plant's ability to complete kernel fill. Lr17a, a leaf rust resistance gene in wheat, is present in many wheat varieties available today. The objective of this research was to identify molecular markers linked to Lr17a. Microsatellite-markers were used on a mapping population of 161 F-2 lines made from a cross of Chinese Spring and Thatcher-Lr17a. Capillary fragment analysis was performed and eight markers were linked to Lr17a, of which Xgwm614 and Xwmc407 flanked Lr17a at genetic distances of 0.7 and 2.5 cM respectively. An evaluation of cultivars with and without Lr17a, and grown in the midwestern United States, revealed that multiple alleles were present for all markers and little correspondence between alleles of closely linked markers and Lr17a was observed. Therefore, caution must taken when using them for marker-assisted selection.Bremenkamp-Barrett, BFaris, JDFellers, JPCrop Sci.10.2135/cropsci2007.07.0379Molecular mapping of the leaf rust resistance gene Lr17a in wheat2008Leaf rust, caused by Puccinia triticina Eriks., is an important foliar disease of common wheat (Triticum aestivum L.) worldwide. Pyramiding several major rust-resistance genes into one adapted cultivar is one strategy for obtaining more durable resistance. Molecular markers linked to these genes are essential tools for gene pyramiding. The rust-resistance gene Lr41 from T. tauschii has been introgressed into chromosome 2D of several wheat cultivars that are currently under commercial production. To discover molecular markers closely linked to Lr41, a set of near-isogenic lines (NILs) of the hard winter wheat cultivar Century were developed through backcrossing. A population of 95 BC(3)F(2:6) NILs were evaluated for leaf rust resistance at both seedling and adult plant stages and analyzed with simple sequence repeat (SSR) markers using bulked segregant analysis. Four markers closely linked to Lr41 were identified on chromosome 2DS; the closest marker, Xbarc124, was about 1 cM from Lr41. Physical mapping using Chinese Spring nullitetrasomic and ditelosomic genetic stocks confirmed that markers linked to Lr41 were on chromosome arm 2DS. Marker analysis in a diverse set of wheat germplasm indicated that primers BARC124, GWM210, and GDM35 amplified polymorphic bands between most resistant and susceptible accessions and can be used for marker-assisted selection in breeding programs.Sun, XCBai, GHCarver, BMol. Breed.10.1007/s11032-008-9237-8Molecular markers for wheat leaf rust resistance gene Lr412009The leaf rust resistance gene Lr47 confers resistance to a wide spectrum of leaf rust strains. This gene was recently transferred from chromosome 7S of Triticum speltoides to chromosome 7A of hexaploid wheat Triticum aestivum. To facilitate the transfer of Lr47 to commercial varieties, the completely linked restriction fragment length polymorphism (RFLP) locus Xabc465 was converted into a PCR-based marker. Barley clone ABC465 is orthologous to the type-I wheat sucrose synthase gene and primers were designed for the conserved regions between the two sequences. These conserved primers were used to amplify, clone and sequence different alleles from ir: speltoides and T. aestivum. This sequence information was used to identify the T. speltoides sequence, detect allele-specific mutations, and design specific primers. Cosegregation of the PCR product of these primers and the T. speltoides chromosome segment was confirmed in four backcross-populations. To complement this dominant marker, a cleavage amplified polymorphic sequence (CAPS) was developed for the 7A allele of Xabc465. This CAPS marker is useful to select homozygous Lr47 plants from F-2 or backcross-F-2 segregating populations, and in combination with the T- speltoides specific primers is expected to facilitate the deployment of Lr47 in new bread wheat varieties.Helguera, MKhan, IADubcovsky, JTheor. Appl. Genet.10.1007/s001220051524Development of PCR markers for wheat leaf rust resistance gene Lr472000The leaf rust resistance gene Lr47 confers resistance to a wide spectrum of leaf rust strains. This gene was recently transferred from chromosome 7 S of Triticum speltoides to chromosome 7 A of hexaploid wheat Triticum aestivum. To facilitate the transfer of Lr47 to commercial varieties, the completely linked restriction fragment length polymorphism (RFLP) locus Xabc465 was converted into a PCR-based marker. Barley clone ABC465 is orthologous to the type-I wheat sucrose synthase gene and primers were designed for the conserved regions between the two sequences. These conserved primers were used to amplify, clone and sequence different alleles from T speltoides and T aestivum. This sequence information was then used to identify the T speltoides sequence, detect allele-specific mutations, and design specific primers. Cosegregation of the PCR product of these primers and the T speltoides chromosome segment was confirmed in four backcross-populations. To complement this dominant marker, a cleavage amplified polymorphic sequence (CAPS) was developed for the 7 A allele of Xabc465. This CAPS marker is useful to select homozygous Lr47 plants from F-2, or backcross-F-2 segregating populations, and in combination with the T speltoides-specific primers is expected to facilitate the deployment of Lr47 in new bread wheat varieties.Helguera, MKhan, IADubcovsky, JTheor. Appl. Genet.10.1007/s001220051397Development of PCR markers for the wheat leaf rust resistance gene Lr472000Leaf rust caused by the fungus Puccinia triticina is one of the most important diseases of wheat (Triticum aestivum) worldwide. The use of resistant wheat cultivars is considered the most economical and environment-friendly approach in controlling the disease. The Lr38 gene, introgressed from Agropyron intermedium, confers a stable seedling and adult plant resistance against multiple isolates tested in Europe. In the present study, 94 F(2) plants resulting from a cross made between the resistant Thatcher-derived near-isogenic line (NIL) RL6097, and the susceptible Ethiopian wheat cultivar Kubsa were used to map the Thatcher Lr38 locus in wheat using simple sequence repeat (SSR) markers. Out of 54 markers tested, 15 SSRs were polymorphic between the two parents and subsequently genotyped in the population. The P. triticina isolate DZ7-24 (race FGJTJ), discriminating Lr38 resistant and susceptible plants, was used to inoculate seedlings of the two parents and the segregating population. The SSR markers Xwmc773 and Xbarc273 flanked the Lr38 locus at a distance of 6.1 and 7.9 cM, respectively, to the proximal end of wheat chromosome arm 6DL. The SSR markers Xcfd5 and Xcfd60 both flanked the locus at a distance of 22.1 cM to the distal end of 6DL. In future, these SSR markers can be used by wheat breeders and pathologists for marker assisted selection (MAS) of Lr38-mediated leaf rust resistance in wheat.Mebrate, SAOerke, ECDehne, HWPillen, KEuphytica10.1007/s10681-007-9615-zMapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers2008Leaf rust of wheat (Triticum aestivum L.) caused by the fungus Puccinia triticina (formerly P. recondita f. sp. tritici), is one of the most important foliar diseases of this crop. Lr37 rust resistance gene, which confers resistance in wheat against leaf rust, was introgressed into cultivated wheat from Aegilops ventricosa Tausch. Rust races with virulence to Lr37 have been identified in different countries, but it still provides resistance to a wide range of races and is useful in combination with other resistance genes. There are no reports about the presence, frequency and origin of Lr37 in Argentinean wheat cultivars. In this work, we analyzed 88 registered Argentinean wheat cultivars developed by different breeding companies and institutions during the last 15 years by means of a molecular marker which is diagnostic of the 2NS-2AS translocation which carries Lr37. Only 4 cultivars showed the amplification product associated with this chromosome fragment. These four cultivars which carry the translocated 2NS-2AS chromosome were registered by the same breeding company during the last seven years and all of them have European germplasm in their genealogy. To the best of our knowledge this is the first report of the presence of Lr37 in registered South American cultivars.Bulos, MEcharte, MSala, CElectron. J. Biotechnol.10.2225/vol9-issue5-fulltext-14Occurrence of the rust resistance gene Lr37 from in Argentine cultivars of wheat2006Leaf rust of wheat, caused by Puccinia triticina, is an important disease throughout the world. The adult plant leaf rust resistance gene Lr48 reported in CSP44 was previously mapped in chromosome 2B, but the marker-gene association was weak. In this study, we confirmed the location of Lr48 to be in the short arm of chromosome 2B and identified closely linked markers suitable for use in breeding. The CSP44/WL711 recombinant inbred line (RIL) population (90 lines) showed monogenic segregation for Lr48. Twelve resistant and 12 susceptible RILs were used for selective genotyping using an iSelect 90K Infinium SNP assay. Closely linked SNPs were converted into Kompetitive allele-specific primers (KASP) and tested on the parental lines. KASP markers giving clear clusters for alternate genotypes were assayed on the entire RIL population. SNP markers IWB31002, IWB39832, IWB34324, IWB72 894 and IWB36920 co-segregated with Lr48 and the marker IWB70147 was mapped 0.3 cM proximal to this gene. Closely linked KASP markers were tested on a set of Australian and Nordic wheat genotypes. The amplification of SNP alleles alternate to those linked with Lr48 in the majority of the Australian and Nordic wheat genotypes demonstrated the usefulness of these markers for marker-assisted pyramiding of Lr48 with other rust resistance genes.Nsabiyera, VQureshi, NBariana, HSWong, DForrest, KLHayden, MJBansal, UKMol. Breed.10.1007/s11032-016-0488-5Molecular markers for adult plant leaf rust resistance gene Lr48 in wheat2016